Abstract
AbstractElectron‐dense arsenic inclusions appeared in the nucleus of parenchymal hepatocytes from fish exposed to arsenate, but were absent in fish exposed under identical conditions to solutions lacking arsenic. Images of these inclusions were compared using conventional transmission electron microscopy (CTEM), scanning transmission electron microscopy (STEM), and high‐voltage electron microscopy (HVEM). Stereo pairs from the same individual inclusions were examined using each of these methods to provide a more complete understanding of their three‐dimensional organization and to evaluate the relative merits of each technique in the study of similar electron‐dense structures.Comparable results were obtained with the three types of instrumentation. Although HVEM is the technique of choice for the analysis of three‐dimensional images of such high electron‐dense structures, STEM proved to be a good alternate technique for the selection and general evaluation of samples in preparation for HVEM.
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