Abstract
There seem to be a certain degree of reluctance in accepting ILP-based methods as part of the range of molecular markers that are classically used for plant genotyping. Indeed, since DNA polymorphism results from difference in length of fragments amplified from specific gene loci, not anonymous sequences, the number of markers that can be generated is sometime inadequate for classical phylogeny studies. Yet, ILP-based markers have many other useful advantages that should not go neglected. We support this statement by presenting a large variety of data we have been collecting for a long while regarding the use of cTBP, an ILP marker based on difference in length of the introns present within the members of the plant beta-tubulin gene family.
Highlights
At the time of their discovery, in the late 1970s, eukaryotic nuclear introns were considered useless, genetically inert DNA sequences, a sort of genomic parasites
Intron Length Polymorphism Detected by the TBP/cTBP Method
Horse TBP represents one of such combinations allowing for the amplification of the whole beta-tubulin genomic region that encompassed the two introns [35]
Summary
At the time of their discovery, in the late 1970s, eukaryotic nuclear introns were considered useless, genetically inert DNA sequences, a sort of genomic parasites. Diversity 2010, 2 of selfish or junk DNA [1,2] As such, their evolution was considered under minimal selective constraints and assumed to be in accordance with the neutral theory of sequence evolution. They actively participate to the control of gene expression, through the mechanism of alternative splicing, that represents a key mechanism allowing for the production of different RNA molecules from the same gene locus, but through a variety of other mechanisms that are being progressively unraveled [3,4]. Introns can control level and site of gene expression through the mechanisms of Intron
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