Abstract
Herbarium specimens have become a major source of information in molecular research of biodiversity. However, getting good DNA samples from old herbarium specimens is still a challenge. The purpose of this project is to test different DNA extraction methods for old material from herbaria that often exhibit high DNA fragmentation. We compared a CTAB-based DNA extraction that is followed by a clean-up with paramagnetic beads with a modified NucleoSpin Plant II protocol, based on silica columns. Our results demonstrate that silica column-based methods have less problems with contamination by polysaccharide and polyphenolic compounds. Taking practical considerations into account, the column-based method is better especially when trying to reduce the amount of leaf tissue used since handling with a tiny pellet makes CTAB difficult.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
