Abstract
BackgroundRahnella aquatilis is ubiquitous and its certain strains have the applicative potent as a plant growth-promoting rhizobacteria. R. aquatilis HX2 is a biocontrol agent to produce antibacterial substance (ABS) and showed efficient biocontrol against crown gall caused by Agrobacterium vitis on sunflower and grapevine plants. The regulatory network of the ABS production and biocontrol activity is still limited known.Methodology/Principal findingsIn this study, a transposon-mediated mutagenesis strategy was used to investigate the regulators that involved in the biocontrol activity of R. aquatilis HX2. A 366-nt noncoding RNA CsrB was identified in vitro and in vivo, which regulated ABS production and biocontrol activity against crown gall on sunflower plants, respectively. The predicted product of noncoding RNA CsrB contains 14 stem-loop structures and an additional ρ-independent terminator harpin, with 23 characteristic GGA motifs in the loops and other unpaired regions. CsrB is required for ABS production and biocontrol activity in the biocontrol regulation by a two-component regulatory system BarA/UvrY in R. aquatilis HX2.Conclusion/SignificanceThe noncoding RNA CsrB regulates BarA-dependent ABS production and biocontrol activity in R. aquatilis HX2. To the best of our knowledge, this is the first report of noncoding RNA as a regulator for biocontrol function in R. aquatilis.
Highlights
All organisms contain a wealth of noncoding regulatory RNAs that function in a variety of cellular processes [1]
R. aquatilis HX2 is a biocontrol agent to produce antibacterial substance (ABS) and showed efficient biocontrol against crown gall caused by Agrobacterium vitis on sunflower and grapevine plants
Most of the known ncRNAs are divided into two categories: cis-encoded ncRNAs that are transcribed in the antisense orientation to their target protein-encoding mRNA, and trans-encoded ncRNAs that are transcribed from intergenic regions with multiple targets [3]
Summary
All organisms contain a wealth of noncoding regulatory RNAs (ncRNAs) that function in a variety of cellular processes [1]. The ncRNAs play key roles in many physiological and adaptive responses in bacteria. The ncRNAs act at the post-transcriptional level and utilize less energy and time for synthesis and turnover [2]. It is more advantageous that bacteria use ncRNA-mediated responses than the regulation by protein transcriptional factors in the rapidly changing environments [3]. Rahnella aquatilis is ubiquitous and its certain strains have the applicative potent as a plant growth-promoting rhizobacteria. R. aquatilis HX2 is a biocontrol agent to produce antibacterial substance (ABS) and showed efficient biocontrol against crown gall caused by Agrobacterium vitis on sunflower and grapevine plants. The regulatory network of the ABS production and biocontrol activity is still limited known
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