CSN1S2 protein of goat milk inhibits the decrease of viability and increases the proliferation of MC3T3E1 pre-osteoblast cell in methyl glyoxal exposure

Abstract

ObjectiveTo investigate whether the CNS1S2 protein of goat milk is able to inhibit the toxicity of methyl glyoxal (MG) towards MC3T3E1 pre-osteoblast cells. MethodsAt confluency, pre-osteoblast cells were divided into five groups which included control (untreated), pre-osteoblast cells exposed to 5 µmol/L MG, pre-osteoblast cells exposed to MG in the presence of CSN1S2 protein at doses of 0.025, 0.050, and 0.100 mg/L, respectively. Analysis of reactive oxygen species was done with 2,7-dichlorodihydrofluorescein diacetate fluorochrome. The proliferation and viability of MC3T3E1 cells were measured by trypan blue staining. Malondialdehyde analysis was done colorimetrically. ResultsCell's viabilities were significantly lower in MG+0.050 mg/L CSN1S2 protein of goat milk compared to MG group (P<0.05). MG+0.100 mg/L CSN1S2 protein of goat milk significantly increased the cells viability compared to MG group (P<0.05). The levels of proliferation were significantly higher in MG+0.100 mg/L CSN1S2 protein of goat milk compared to control group and all treatment groups, respectively (P<0.05). ConclusionsHigh dose of CSN1S2 protein of goat milk (0.100 mg/L) in high MG environment inhibits the decrease of viability due to the increases of the proliferation of MC3T3E1 pre-osteoblast cell.