CS<SUB>2</SUB>の代謝に関する研究 : グルタミン酸とCS<SUB>2</SUB>との化合物の代謝

Abstract

Concerning CS2 metabolism in the body, the balance sheet of inhaled and excreted CS2, and also the end products of its metabolism excreted in the urine have to some extent been made clear. Intermediate change of CS2, however, is not yet well known except chemical combination of CS2 with protein and α-amino-acid. In this report, metabolism of compounds of CS2 with protein or amino-acids was studied in rabbits by a method of determining the amount of reversibly combined CS2 Compounds of CS2 with protein or amino-acid have a characteristic nature of liberating free CS2, when pH of the solution of these compounds is adjusted to 2 or less. Therefore, the amount of free CS2 liberated by adjustment of pH is a measure of reversibly combined CS2. The results obtained are as follows. 1. In the fluid media of liver, kidney and brain slices to which CS2 was added, sulfate was not demonstrated to increase when compared with the control. 2. In the course of 1.5 hours following subcutaneous injection of CS2, reversible free CS2 was not proved to exist in the blood of rabbits. Accordingly, compounds of CS2 with protein or amino-acid may not be retained as such in the body. 3. When compounds of CS2 with glutamic acid (CS2-G) or with diethylamine (CS2-D) were injected into the rabbit, these were not detected in the blood in 1.5 hours after the injection. Thus CS2-G or CS2-D was considered to change readily into other forms. 4. CS2-G compound was considered to react with some constituents of the blood, since CS2-G added to the hemolyzed blood solution could not be recovered. 5. It was revealed that CS2-G reacted with hemoglobin forming some compounds which do not liberate free CS2, when pH of the reaction medium was adjusted to 2 or less. 6. Moderately diluted serum in saline solution did not seem to react with CS2-G, whereas larger volume of such solution reacted with CS2-G. 7. Tyrode's solution or glutathione solution was not found to react with CS2-G.