CS and US duration effects in one-trial simultaneous fear conditioning as assessed by conditioned suppression of licking in rats

Abstract

In three experiments, rats received a single presentation of an auditory conditioned stimulus (CS) beginning simultaneously with an electric grid-shock unconditioned stimulus (US). Later, the CS was presented while the rats licked a drinking tube for water, and CS-elicited suppression of licking was taken as an index of the excitation conditioned to the CS. It was found that conditioning increased as a joint function of the duration of CS-US overlap and US duration. The evidence suggested that weak conditioning due to a brief CS-US overlap could be increased by extending the US beyond CS termination. Extending CS duration beyond US termination, however, did not strengthen conditioning; indeed, extending the CS 60 sec beyond US termination weakened conditioning significantly. It is suggested that these results shed light on a discrepancy in the recent literature on simultaneous conditioning.