Crystalloscopic analysis in detection of saliva metabolome specialties in elderly people

Abstract

The purpose of this study was to assess the features of saliva crystallization in middle-aged and elderly people. The study included middle-aged (45-60 years old; n=63) and elderly (over 60 years old; n=78) men who signed an informed consent to participate. Crystalloscopic analysis of saliva in order to characterize the metabolic characteristics of the body and the composition of biological fluids. The intrinsic crystallization of mixed saliva was studied using the method of classical crystalloscopy. The structure index, crystallizability, facia destruction degree and the clearity of the marginal protein zone were determined. Each of the parameters was expressed on a 5-point scale. In general, the study made it possible to establish the presence of shifts in the crystallogenic properties of mixed saliva in the older age group (over 60 years old) relative to middle-aged people. Thus, the features of the crystalloscopic picture of biological fluid in elderly subjects included a decrease in the total crystallogenic potential of biological fluid, which was manifested in a decrease in crystallizability and the structural index of dehydrated saliva facies. In addition, the involutive shifts of the crystallograms were represented by an increase in the degree of destruction of facies and the expansion of the marginal zone of micropreparations. The revealed transformations of the crystallogenic properties of the biological medium allow us to speak about age-dependent characteristics of the component composition and physico-chemical parameters of saliva and, therefore, indirectly describe the involutive changes in the metabolome of this biological fluid.