Crystallographic evidence for β-cyclodextrin inclusion complexation facilitating the improvement of antioxidant activity of tea (+)-catechin and (−)-epicatechin

Abstract

Single-crystal X-ray analysis to 0.6Å resolution using synchrotron radiation of the inclusion complexes of β-cyclodextrin (β-CD) with (+)-catechin (CA) and (−)-epicatechin (EC) has been carried out to gain atomistic insight into how the inclusion complexation helps to improve tea catechin antioxidant activity. The β-CD–CA complex crystallizes in the monoclinic P21 space group, whereas the β-CD–EC complex crystallizes in both the monoclinic P21 and triclinic P1 space groups. In comparing the inclusion structures, the β-CD–EC dimeric structure having higher numbers of OH⋯O hydrogen bonds combined with π⋯π interactions is associated with its greater interaction energy, as derived from DFT/B3LYP single-point energy calculations. Detailed structural analysis of the CA, EC conformation and inclusion geometry reveals that the interplay of the intermolecular OH⋯O hydrogen bond stabilized catechol moiety and the protection of OH groups in the β-CD cavity plays a key role in the improvement of antioxidant capacity upon inclusion complexation.