Crystallization Kinetics of Calcium Oxalate in Fresh, Minimally Diluted Urine: Comparison of Recurrent Stone Formers and Healthy Controls in A Continuous Mixed Suspension Mixed Product Removal Crystallizer

Abstract

A reproducible method has been developed for studying calcium oxalate crystallization from fresh, minimally diluted (92%) urine with the mixed suspension mixed product removal continuous crystallization technique. All samples were adjusted to give the same starting calcium and oxalate concentrations. Twenty-one recurrent male stone formers were compared with twenty-two healthy controls. There was no difference in crystal growth rates but crystal nucleation rates were much higher in the control group (p = 0.003). Using growth rate and nucleation rate results, the amount of crystalline material in suspension was shown to be lower in the urine from stone formers, and therefore the equilibrium supersaturation in the crystallizer was lower in the control group (p = 0.001). We propose that the ability of a healthy person’s urine to maintain a lower supersaturation is a crucial protective factor distinguishing non-stone formers from stone formers.