Crystallization and preliminary X-ray investigation of phosphoribosylaminoimidazolesuccinocarboxamide synthase from the yeast Saccharomyces cerevisiae

Abstract

Crystals of phosphoribosylaminoimidazolesuccinocarboxamide synthase (EC 6.3.2.6) from the yeast Saccharomyces cerevisiae were grown by the vapor diffusion hanging-drop technique, using ammonium sulfate as the precipitant. The crystals had dimensions up to 1.2 mm. X-ray diffraction experiments indicated a space group of P2 1 2 1 2 1 and unit cell parameters of a = 62.3 A ̊ , b = 63.5 A ̊ and c = 80.9 A ̊ , with one molecule in the asymmetric unit. Native data have been collected to 2.5 Å resolution.