Abstract
Chaperonin-60 (cpn60) from Paracoccus denitrificans has been crystallized using polyethyleneglycol(PEG) 6000 and LiCl as precipitants. The crystals diffract up to 3.0 Å resolution by using synchrotron radiation. They belong to the tetragonal space group P4 22 12 with unit cell parameters of a = b = 284 A ̊ , c = 152 A ̊ . Seven subunit molecules of cpn60 seem to exist in the asymmetric unit of the crystal. A complete diffraction data set was collected using a Weissenberg camera system attached to the synchrotron radiation source and merged up to 3.2 Å resolution. Self-rotation functions calculated show the existence of a local 7-fold axis, suggesting the 7-fold symmetry ring assembly.
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