Crystallization and crystallographic data for new forms of thymidylate synthase from Lactobacillus casei

Abstract

Several new crystal forms of thymidylate synthase (5,10-methlenetetrahydrofolate: dUMP C-methyltransferase; EC 2.1.1.45) were obtained by controlled pH change. In the crystals the dimeric molecule has a 2-fold symmetry axis coinciding with crystallographic symmetry. The crystals scatter to at least 2.7 Å resolution in the synchrotron X-ray beam and appear to be suitable for high-resolution X-ray diffraction analysis. The crystals were successfully derivatized and preliminary results are reported for the covalent inhibitory ternary complex of thymidylate synthase, 5-fluoro-2′-deoxyuridylate and 5,10-methylenetetrahydrofolate.