Abstract

Two different crystal forms of the B800–850-antenna complex from Rhodopseudomonas acidophila strain 10050 have been grown. This complex is an integral membrane protein and is isolated as an oligomeric assembly with a molecular weight of approximately 84 kDa. This assembly contains six α/β apoprotein pairs, 18 molecules of bacteriochlorophyll a and nine molecules of carotenoid. The first crystal form has dimensions unit cell a = b = 75·8 A ̊ , c = 97·5 A ̊ with the space group P4 and diffracts to a resolution of 12·0 Å. The second crystal form is rhombohedral with dimensions unit cell a = 121·1 A ̊ , α = 60° , space group R32 and diffracts to a resolution of 3·5 Å. Native data have been processes in both cases, to an R merge value of 9·0 to 11·0%. The X-ray data suggest that the asymmetric unit, in both crystal forms, contains one 84 kDa antenna complex.

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