Abstract
Crystal Structure of S-Adenosylmethionine Synthetase
Highlights
There are a myriad of reactions in which methyl groups are transferred from a few types of methyl donors to a wide variety of methyl acceptors
Among biological methyl group donors, S-adenosylmethionine (AdoMet),1 discovered by Cantoni in 1953 [1], is the most widely used, while 5-methyltetrahydrofolic acid, methylcobalamin, and betaine are involved in far fewer methylation reactions
In addition to its role as a methyl donor, AdoMet undergoes decarboxylation to generate the propylamine donor in the biosynthesis of the polyamines, spermine and spermidine, which are widely distributed in nature and appear to be involved in cellular proliferation [9]
Summary
32.1 32.7 35.3 a DIP100S, the diffraction data were measured at the University of Kansas using a DIP100S imaging plate x-ray diffractometer; MARK-III, the diffraction data were measured at the University of California at San Diego using the MARK-III multiwire area detractor x-ray diffractometer. b Rsym ϭ ⌺ I ϪI /⌺͗I. c I/(I) in 3.0 –3.1 Å resolution range. d RFD ϭ ⌺ FPH Ϫ FP /⌺FP. C I/(I) in 3.0 –3.1 Å resolution range. The SeMAT and MAT-U data were cut off at 3.5 Å and 4.0 Å resolution, respectively. F Phasing power ϭFH/͗E; the root mean square heavy atom structure factor amplitude divided by the residual lack of closure error. G FOM of SIR ϭ figure of merit of single isomorphous replacement. The phasing statistics listed in the table are for 4.0 Å resolution data. The completeness of the data at 4.0 Å resolution level is more than 98.7%. H FOM of MIR ϭ figure of merit of multiple isomorphous replacement (Se and U derivatives). The value after solvent flattening phase refinement is given in parentheses. The value after solvent flattening phase refinement is given in parentheses. i R ϭ ⌺ Fo Ϫ Fc /⌺Fo
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
