Abstract
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme. Here we report the crystal structures of the enzyme in complex with dimethylglycine and folinic acid. The α subunit is composed of two domains, contains NAD +, and binds folinic acid. The β subunit contains dimethylglycine, FAD, and FMN, and these flavins are approximately 10 Å apart. The γ subunit is in contact with two domains of α subunit and has possibly a folate-binding structure. The δ subunit contains a single atom of zinc and has a Cys 3His zinc finger structure. Based on the structures determined and on the previous works, the structure–function relationship on the heterotetrameric sarcosine oxidase is discussed.
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