Crystal structure of an Escherichia coli tRNA Gly microhelix at 2.0 Å resolution

Abstract

tRNA identity elements determine the correct aminoacylation by the cognate aminoacyl-tRNA synthetase. In class II aminoacyl tRNA synthetase systems, tRNA specificity is assured by rather few and simple recognition elements, mostly located in the acceptor stem of the tRNA. Here we present the crystal structure of an Escherichia coli tRNA Gly aminoacyl stem microhelix at 2.0 Å resolution. The tRNA Gly microhelix crystallizes in the space group P3 221 with the cell constants a = b = 35.35 Å, c = 130.82 Å, γ = 120°. The helical parameters, solvent molecules and a potential magnesium binding site are discussed.