Crystal structure and protective effect in osteoarthritis of Co(II)-mediated pyridinium-bearing coordination polymer

Abstract

By using a mixed-ligand approach, a new Co(II)-mediated pyridinium-bearing coordination polymer, {[Co(BCbpe)(ipa)]·3H2O}n (1), has been prepared by reaction of Co(NO3)2·6H2O) and isophthalic acid (H2ipa) in the presence of pyridinium chloride 1-(4-carboxybenzyl)4-[2-(4-pyridyl)-vinyl] (HBCbpeCl) in EtOH/H2O (V:V = 1:1). Furthermore, a green hand grinding technique has been implemented to reduce the particle size of 1 to generate nanosheets of 1 with good water dispersivity. The post-traumatic osteoarthritis (OA) model was constructed and the in vivo protective effect of nano 1 was evaluated. First, the inflammatory level of OA rats was measured by ELISA detection. Second, for further detection, the real-time RT-PCR was carried out to measure the relative expression of caspase-1, nlrp3 as well as il-1β inflammatory factors. Finally, molecular docking analysis of the complex indicated that all of the proposed drug candidates showed desirable drug-like criteria.