Abstract
The Malpighian tubules of the silkworm ( Bombyx mori) produce crystals of calcium oxalate. The crystals are birefringent and easily seen in the optical microscope using polarized light. The first stage of crystal development, visible at the fine structural level, is the appearance of patches of matrix in the tubule lumen. The matrix is closely associated with the cell surface. Gradually more matrix appears and serves as a template for the deposition of crystalline calcium oxalate. The tubule lumen is eventually filled with mature crystals that are released when the larval cuticle is shed. This cycle repeats during each intermolt period.
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