Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study.

Abstract

Cryptosporidium is a leading cause of diarrheal mortality in children in Africa and Asia. Despite the public health significance of this parasite, its molecular epidemiology and circulation in Guinea remain poorly understood. Therefore, this study aimed to determine the prevalence and genotype distribution of Cryptosporidium in the Guinean general population. To achieve this, fecal samples were collected from 834 individuals, both with and without digestive disorders, at two hospitals in Conakry. The presence of the parasite in the stool samples was detected using nested PCR targeting the SSU rDNA gene, followed by sequencing of the PCR products for genotyping of the isolates. The PCR-based prevalence was 0.12% for the whole cohort, and 0.2% among adults. The low frequency of Cryptosporidium observed in the current study is thus consistent with the prevalence of this parasite already reported in certain other African countries. The species identified in the positive samples was Cryptosporidium hominis. This study is the first to report the prevalence of Cryptosporidium in the general population of Guinea. Given the potential of this parasite to cause life-threatening diarrhea, further studies are needed to clarify the epidemiology of Cryptosporidium in this country.