Abstract
Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3g/L and EPS synthesis 4.3g/L in 144h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH3, 25°C and low aeration (1% < pO2 < 10%) were advantageous, while cell growth optimum was at pH6, 20°C, and high aeration (pO2 > 30%). As medium pH changed from pH3 to pH6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15°C) increased glucose content in EPS. Aeration intensity and time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85%. In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats. The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
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