CryoSeal™ System, a New Device for Generating Cryoprecipitate from Plasma

Abstract

The CryoSeal™ System (CSS; Thermogenesis Corp.) is a device that can prepare freeze–thaw cryoprecipitate (Cryo) generated from plasma in ∼1 h. Traditional blood bank preparations of Cryo contain high concentrations of antihemophilic factor (plasma coagulation factor VIII) and fibrinogen, but require several days, numerous pieces of laboratory equipment, and considerable staff handling to prepare (1). We investigated whether CSS-prepared material is substantially equivalent in factor VIII and fibrinogen contents to the traditionally prepared Cryo material. Whole blood drawn from volunteer donors (n = 16) at the Sacramento Medical Foundation Blood Center (SMFBC), with use of citrate–phosphate–dextrose–adenine anticoagulation, was centrifuged at 2500 g for 5 min at 4 °C within 6 h of collection. The units of plasma were then used for preparation of Cryo in the CSS. The CSS consists of a thermodynamic device featuring a temperature-controlled rocker plate and a clear, plastic, single-use plasma-processing container that rests on the rocker …