Cryopreserved and Lyophylized Amniotic Fluids using Levine’s Conservation Model: A Breakthough in the Process of Wound Healing

Abstract

Healing restores continuity and form through cell replication; hence, conserving structural integrity. Early detection and management of wounds by nurses reduces tissue destruction, which also conserves structural integrity. In response to the worldwide pressing problem of chronic wounds in the healthcare delivery system, the researcher aims to provide effective intervention to preserve the structural integrity of the person. The cryopreserved and lyophilized amniotic fluids are sophisticated processes in formulating a drug in the form of ointment. These methods preserved the cell structure of amniotic fluid from term fetus and embed into two (2) concentrations (1.5 % and 1.0 %) of an absorption-based ointment. The total protein concentration of Cryopreserved and Lyophilized Amniotic Membrane in full term fetus was determined by the Biuret and Bradford methods, which are based on UV-visible spectroscopy. The percentages of protein presence in 9.5 mg (mass total sample) of Amniotic fluids ranges between 14.77 – 14.46 % in Bradford method, while slightly lower to 13.78 – 13.80 % concentration in Biuret method, respectively. Bradford method evidently showed higher sensitivity for proteins than Biuret test. Overall, the amniotic membrane, composed principally of proteins, proved its healing abilities. Levine’s conservation model emphasized that it was the nurse’s duty to promote healing as well as protect the patient from external factors that may contribute to physical breakdown and complications. Keywords - Wounds, Healing, Amniotic membrane ointments