Abstract
Cryopreservation of semen of Thymallus thymallus and of Hucho hucho was investigated with a method that was originally developed for Oncorhynchus mykiss. Assessments of fertilization rate were used to establish the type and concentration of cryoprotectant, freezing rates and thawing conditions for the two species. In both H. hucho and T. thymallus, 10% methanol was the most effective cryoprotectant, followed by a 5% DMSO-1% glycerol mixture, 10% DMSO, 10% n, n-dimethylacetamide and 5% glycerol. Using an open system and 0.5ml straws, freezing of semen was optimal 1.5 cm above the level of liquid nitrogen (at − 110 ± 2 °C) and thawing was best in a 25 °C water bath for 30s. Post-thaw fertilization rates were 90–100% of control with fresh semen at sperm/egg ratios of (1.2–1.6) × 10 6 spermatozoa per egg in T. thymallus and at sperm/egg ratios of (4.3–5.5) × 10 6 spermatozoa per egg in H. hucho.
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