Cryopreservation of semen of initial forms and chimeras of birds of two breeds: Sussex light and Poltava clay

Abstract

Purpose: evaluation of the quality of seed chimer and source forms before and after cryopreservation.Materials and methods. For research, the breed of Sussex chickens and Poltava clay. Using these rocks, chimeras were obtained by the method of transplantation of blastodermal cells. An assessment of the quality of sperm of the roosters was carried out under a microscope at a temperature of 42 degrees. At various times, the activity of native sperm and its concentration was determined. Cryoconservation was carried out in small granules. The defrosting of the granules was produced on a heated metal plate at a temperature of 60 ° C.Results. A comparative analysis of the volume of the ejaculate showed that Himer on average it was somewhat larger than that of the initial forms. The activity of sperms in native genital cells was sufficiently high in all studied samples, and was 80% or more. The concentration of sperm retained the same trend and practically did not differ from each other in all studied samples. The activity of frozen-fatty seed of the initial breeds and their chimer was significantly different. The maximum activity was observed in the Sussex breed and reliably below (p≤ 0.01) it has the remaining experimental groups. The activity of frozen-fatty seed turned out to be equal in chimeric organisms and Poltava clay, although Himer recipients were sussexes. It should be noted that the swing of oscillations according to the degree of activity of frozen-fatty seed in chimeric birds was significant (from 10 to 40 points), and the number of observations is small.Conclusion. The study assess the quality of seed chimer and the source forms before and after cryopreservation. It was demonstrated that after defrosting the activity of frozen-fatty seed of the initial rocks and their chimer was different.