Abstract
Department of Marie Biomaterials and Aquaculture, Pukyong National University, Busan 608-737, Korea This study demonstrated that cryopreserved semen from dead fish can be used for seedling production. Yellow croakers, Larimichthys polyactis, were killed and stored at temperatures of 20°C or 0°C for 6 hours. At 2 hour intervals, semen from these fish was collected using abdominal pressure and evaluated for spermatozoa motility and semen cryopreservation. Semen collected after 6 hours from dead fish stored at 0°C could be cryopreserved and attained fertilization and hatching rates of 15.0±1.2% and 14.8±1.6%, respectively. This study suggests that germ cells such as the semen of dead fish can be cryopreserved and utilized in the restoration of a species. Key words: Yellow croaker, Larimichthys polyactis, Semen, Cryopreservation, Dead fish
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