Abstract
800x600 Shoot tips excised from in vitro plants of Rosa x hybrida L., cvs. Ioana, Mariana and Vulcan were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification methods. Shoot tips (2-3 mm in length) were excised, encapsulated in natrium alginate beads, precultured in sucrose (0.25, 0.5, 0.75 M) for 24 h and either desiccated in laminar air flow (up to 5 h) or dehydrated with a vitrification solution (up to 220 min) prior to direct immersion in liquid nitrogen. The highest shoot development (72% for cv. Vulcan, 69% for cv. Mariana and 66% for cv. Ioana) after storage in liquid nitrogen was obtained after using the encapsulation-vitrification protocol. Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
