Abstract
Abstract Seeds of Pyrostegia venusta (Ker Gawl.) Miers) were desiccated at 25 °C on silica gel for 0 h (T0), 7 h (T1) and 24 h (T2), reaching moisture contents of 6.5%, 4.4% and 3.8%, respectively. Seeds were frozen rapidly in liquid nitrogen (+LN) at -263 °C.min.-1 and after 72 h, they were thawed slowly at room temperature (25 ± 2 °C) at a speed of 5 °C.min.-1. Seed germinability evaluation before (-LN) and after freezing (+LN) was conducted at 25 °C, on paper roll substrate, and germinated at 16 hrs light/8 hrs dark, normal seedling counts for 38 days. The germinative percentages were 88% (T0-LN), 98% (T0+LN), 61% (T1-LN), 95% (T1+LN), 78% (T2-LN) and 89% (T2+LN). Mean days for seedling formation were 23 (T0-LN), 22 (T1-LN and T2-LN) and 30 days for seeds exposed to LN. Fast freezing, slow thawing, and the three tested moisture contents, were suitable for cryopreservation of Pyrostegia venusta seeds.
Highlights
Lianescent, herbaceous, shrub and arboreal species of the Bignoniaceae family are widely distributed throughout South America (Ortolani 2007; Camarinha et al 2015)
Considering that seeds with lipid reserves, such as those of P. venusta, present viability loss when stored in cold rooms at -20 °C, the most promising approach to ensure long-term conservation of this species germplasm is cryopreservation in liquid nitrogen, (Michalak et al 2013)
Pyrostegia venusta seeds had an initial mc of 6.5%, which corresponded to the desiccation time T0
Summary
Lianescent, herbaceous, shrub and arboreal species of the Bignoniaceae family are widely distributed throughout South America (Ortolani 2007; Camarinha et al 2015). One of the physiological characteristics of seeds of species of the Bignoniaceae family is that they are short-lived under storage (Aguiar 2010, Tresena et al 2010, Martins et al 2014) This is generally attributed to the instability of lipid compounds present in the bilobed cotyledons of the seeds (Gabrielli 1988, Renó et al 2007). Considering that seeds with lipid reserves, such as those of P. venusta, present viability loss when stored in cold rooms at -20 °C, the most promising approach to ensure long-term conservation of this species germplasm is cryopreservation in liquid nitrogen, (Michalak et al 2013). Cryopreservation techniques are used in an increasing number of gene banks and research facilities around the world (Kalaiselvi et al 2017, Panis 2019)
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