Abstract
In vitro-grown apical meristems of hybrid statice ( Limonium cv. Blue Symphonet) were cryopreserved by three cryogenic procedures; (1) vitrification with encapsulation, (2) vitrification without encapsulation, and (3) a revised encapsulation/dehydration technique. When dehydration tolerance was well developed by preconditioning and cryogenic procedures were well optimized, these three procedures produced nearly the same levels of growth recovery (70–75%). These results support our theory that the acquisition of dehydration tolerance is sufficient for specimens to survive to cryopreservation.
Published Version
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