Abstract
This report deals with a cryopreservation technique and procedure; clinical data concerning the therapy itself are not presented here. A cryopreservation procedure was developed which proved to be effective for large volumes of lymphocytes and stem cells (CFU-c) obtained exclusively from the peripheral blood of man. Viability criteria used for lymphocytes include determination of the number of living cells, absolute recovery and immunologic assays. Stem cell counts and recovery were determined by the semisolid agar colony assay. A specially designed freezing process and a revitalization method using an atomizing system were applied to cell concentrates of patients with different types of cancer. The results indicate that nearly all cell functions of interest are fully preserved and that very high amounts of MNC and CFU-c are obtained after the freeze-thaw cycle. Up to now, units of cryopreserved autologous cells were retransfused to 40 patients.
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