Cryopreservation of Human Adipose Tissues using Human Plasma

Abstract

Human adipose tissue or derived cells have been used clinically in several fields. Animal serum is used in storing and culturing human adipose tissue or derived cells. Thus far, few problems have been associated with these sera in current research fields. However, animal sera may cause some problems in clinical applications because of the toxic viruses and proteins that can be present in animal sera. Human plasma can resolve these problems associated with animal serum. In this study, we investigated the effects of human plasma as a cryopreservation solution for human adipose tissues. We observed that adherent cells derived from adipose tissues were isolated effectively by collagenase treatment. We did not observe any adherent cells when human adipose tissues were preserved without cryopreservation solution at ?20°C. Adherent adipose-derived cells exhibited different growth rates based on the cryopreservation solution and storage temperature. We also did not observe adherent cells when human adipose tissues were preserved with cryopreservation solution at ?20°C and ?70°C. Adherent adipose tissue-derived cells exhibited a similar growth rate as cryopreserved tissues when cryopreserved in 10% dimethyl sulfoxide (DMSO) + 90% fetal bovine serum (FBS) or 10% DMSO + 90% human plasma cryopreservation solution but exhibited a low growth rate when cryopreserved in 10% DMSO + 10% FBS + 80% Dulbecco’s modi?ed Eagle’s medium (DMEM) cryopreservation solution. In conclusion, these results demonstrated that human plasma is useful as a cryopreservation solution for human adipose tissues.