Abstract
Filaments of the brown alga, Scytosiphon lomentaria cultured in our laboratory were cryopreserved at −20 °C using a vitrification procedure. We investigated the effects of different loading solutions (LS), vitrification solutions (VS), loading times, and dehydration times on the viability of cryopreserved filaments of S. lomentaria. The highest survival rate (38.42 %) was achieved when filaments of S. lomentaria were loaded with LS5 for 30 min, dehydrated with VS2 for 30 min at 0 °C, cryopreserved instantly and then kept at −20 °C for 1 month, then rewarmed at 40 °C in a water bath, rinsed with 1.2 M sucrose for 20 min, and cultured in sterile seawater for 2 days. The filaments recovered after cryopreservation showed the same morphology and growth as non-cryopreserved filaments. The recovered filaments were able to produce normal sporangia, which released viable spores. Compared with non-cryopreserved filaments, the cryopreserved filaments produced fewer sporangial branchlets and fewer spores. However, the spores released by cryopreserved filaments of S. lomentaria showed strong vitality and were able to grow into normal, healthy thalli.
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