Abstract
Abstract We developed methods for cryopreserving sperm of channel catfish Ictalurus punctatus and evaluated the use of cryopreserved sperm for reproduction. Five cryoprotectants were evaluated: Methanol, glycerol, dimethyl sulfoxide (DMSO), sucrose, and polyvinylpyrrolidone. We measured the motility of sperm that had been stored at 4°C in three concentrations of cryoprotectants (5%, 10%, 15%) dissolved in a modified Hank's balanced salt solution. All cryoprotectants reduced motility within 6 h; 5% methanol and 5% DMSO caused the smallest reduction. After sperm were frozen at –80°C and stored for 2 d at –196°C, motility was highest (5–10%) in samples cryopreserved with 5% and 10% solutions of methanol. Sperm cells cryopreserved in methanol solutions (5%, 10%, and 15%) were used to fertilize channel catfish eggs from three females. Fertilization ranged from 24% to 97%, and no difference in fertilization success was found between cryopreserved sperm and untreated sperm from the same males. Growth of channel ...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
