Cryopreservation of Cells in 3D Constructs Based on Controlled Cell Assembly Processes

Abstract

Cryopreservation technology plays an important role in conserving three dimensional (3D) constructs containing cells. Besides preserving the characteristics of the construct, it can also save a lot of resources in many aspects, such as cell culture space, culture vessel, culture medium etc. Otherwise, the biological properties of the cells and the 3D geometrical configuration will disappear with the death of cells and breakage of configuration. Consequently, a cryopreservation method for the 3D construct fabricated with a controlled cell assembling technology was studied. 10% dimethylsulfoxide (DMSO) was incorporated into the adipose-derived stem cell (ADSC)/gelatin/ alginate/fibrinogen mixture before assembling. Results indicate that the 3D construct containing cells can be preserved below —80°C for more than 1 week. After the construct underwent the thawing process, cell viability and proliferation ability were regained. This technique holds potential to be used widely in tissue engineering and organ manufacturing fields.