Abstract
Semen extenders containing egg yolk as a cryoprotectant may pose hygienic risks and are difficult to standardize. Although a new generation of semen extenders free of animal ingredients is available, egg yolk-containing extenders are still widely used for cryopreserving semen. The aim of the present study was to compare the effect of using soy lecithin-based extenders, Biociphos and Bioxcell, and egg yolk-based extender on buffalo spermatozoa freezability and fertilizing potentials. Extension of buffalo bull semen in the Bioxcell and the Biociphos extenders significantly increased (P 0.05) were detected between the extenders for the non-return rates. We suggest that consistent with quality standards that should be required for cryoprotectant media, soy lecithin-based diluents might be the best choice as a buffalo semen extender in the future.
Highlights
The process of cryopreserving semen has profound damage effects on spermatozoa, many of which result in sublethal damage to the sperm cells, and subsequent reduction of fertility
Effect of freezing extenders on sperm motility, viability, and acrosomal integrity: Data presented in table 1 revealed that, extension of buffalo semen in Bioxcell and Biociphos extenders enhanced sperm freezability and increased significantly (P
The results of the present study have clearly demonstrated that the soy lecithin-based extenders increased the freezability and the fertilizing potentials of buffalo spermatozoa compared with TRIS- egg yolk extender
Summary
The process of cryopreserving semen has profound damage effects on spermatozoa, many of which result in sublethal damage to the sperm cells, and subsequent reduction of fertility. Glycerol is a preferable cryoprotectant for sperm freezing in most mammals Complex agents such as egg yolk, skim milk, milk and even serum are used in sperm freezing extenders for different species in order to provide maximal cryoprotection for spermatozoa (Holt, 2000). Egg yolk and milk introduce possible sanitary risks (viruses, bacteria and fungi), with the subsequent production of endotoxins capable of damaging the fertilizing capacity of spermatozoa (Bousseau et al, 1998 and Van Wagtendonk-de Leeuw et al, 2000). Previous studies have described how the spermatozoa of a mouse (Storey et al, 1998), goat (Kundu et al, 2000 and Janett et al, 2005) and bovine (Aires et al, 2003) were successfully frozen in a chemically defined extender, but not yet those of buffalo
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