Abstract
Kaempferia galanga L. is an endangered multi-purpose medicinal plant in Family Zingiberaceae, the rhizomes of which are used for several ayurvedic formulations. Encapsulation-dehydration (ED) method was optimized for cryopreservation of shoot tips of K. galanga. Shoot tips (STs) bearing the apical meristem dissected from the established in vitro shoot cultures were preconditioned in MS+0.4 M sucrose prior to encapsulation in calcium alginate and the beads subsequently transferred to MS liquid+0.3 M sucrose for 3 days afterward dehydration inside the laminar airflow for 4 hours upon rapid freezing in LN and rapid thawing produced maximum 62.2% survival and 46.7% regeneration rates. Shoot regeneration was observed from the apical meristems exclusive of intermediary callus phase. The plantlets regenerated from cryopreserved STs transferred to the field were phenotypically analogous with the mother plant.
Highlights
Kaempferia galanga L., a high sought endangered multi-utility medicinal plant of the Family Zingiberaceae (Shirin et al, 2000) is cultivated in Tropical Asia for its aromatic rhizome which is having a wide range of medicinal applications (Sadiman, 1992)
The shoot meristems dissected out from in vitro shoot cultures were preconditioned in MS medium supplemented with 0.4 M sucrose for one day and used for cryopreservation experiments
The results indicate that preculture duration, concentration of sucrose in the medium and optimal moisture content (MC) in the beads are critical factors for cryopreserving K. galanga Shoot tips (STs)
Summary
Kaempferia galanga L., a high sought endangered multi-utility medicinal plant of the Family Zingiberaceae (Shirin et al, 2000) is cultivated in Tropical Asia for its aromatic rhizome which is having a wide range of medicinal applications (Sadiman, 1992). Various uses of aromatic ginger (K. galanga) for health benefits, food and nutritional purposes are well documented (Preetha et al, 2016). Received in revised form: 20 Sep 2021. From Volume 13, Issue 1, 2021, Notulae Scientia Biologicae journal uses article numbers in place of the traditional method of continuous pagination through the volume. The journal will continue to appear quarterly, as before, with four annual numbers
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