Abstract
Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.
Highlights
Aceh cattle has been stated as one of Indonesian beef cattle germplasm by the Ministry of Agriculture of The Republic of Indonesia (Kementan, 2011)
Spermatozoa damage caused by reactive oxygen species (ROS) in the cryopreservation process can be reduced with the addition of natural antioxidants, compounds found in high concentration in the date extract (Al Farsi et al, 2005; Al Juhaimi & Ghafoor, 2014)
The results obtained can be used as a basis of using palm date extract for improving or maintaining the quality of frozen aceh cattle semen
Summary
Aceh cattle has been stated as one of Indonesian beef cattle germplasm by the Ministry of Agriculture of The Republic of Indonesia (Kementan, 2011). Spermatozoa damage caused by ROS in the cryopreservation process can be reduced with the addition of natural antioxidants, compounds found in high concentration in the date extract (Al Farsi et al, 2005; Al Juhaimi & Ghafoor, 2014). This study was done to investigate the quality and DNA spermatozoa integrity of aceh cattle semen diluted in egg yolk tris extender containing different concentrations of palm date extracts. The results obtained can be used as a basis of using palm date extract for improving or maintaining the quality of frozen aceh cattle semen. This might help effort to support genetic improvement and breeding selection of aceh cattle through artificial insemination-based breeding program nationally
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biosaintifika: Journal of Biology & Biology Education
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
