Abstract
Abstract The radioresistance of bovine herpesvirus-1 (BHV-1), commonly known as infectious bovine rhinotracheitis virus (IBRV), suspended in free serum Glasgow-MEM medium and frozen at −78°C was studied. The number of surviving virus at a given dose of gamma-radiation was determined by a plaque assay system. D10 values were calculated before and after removal of cell debris. The D10 values obtained were 4.72 kGy and 7.31 kGy before and after removal of cell debris, respectively. Our results indicate that the inactivated viral particles could be used for vaccine preparation or diagnostic reagents.
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