Abstract
Abstract Crustacean vitellogenesis is a process that involves Vitellin, produced via endoproteolysis of its precursor, which is designated as Vitellogenin (Vtg). The Vtg gene, mRNA and protein regulation involve several environmental factors and physiological processes, including gonadal maturation and moult stages, among others. Once the Vtg gene, mRNAs and protein are obtained, it is possible to establish the relationship between the elements that participate in their regulation, which could either be species-specific, or tissue-specific. This work is a systematic analysis that compares the similarities and differences of Vtg genes, mRNA and Vtg between the crustacean species reported in databases with respect to that obtained from the transcriptome of Callinectes arcuatus, C. toxotes, Penaeus stylirostris and P. vannamei obtained with MiSeq sequencing technology from Illumina. Those analyses confirm that the Vtg obtained from selected species will serve to understand the process of vitellogenesis in crustaceans that is important for fisheries and aquaculture.
Highlights
Crustacea have different sexual systems, each aimed at achieving maximum reproduction efficiency in their specific situation
Ovary maturation comprises the synthesis of yolk protein denoted as Vitellin (Vn), which is the most important source of energy and nutrients for embryo development (Boulangé-Lecomte et al, 2017)
The process of Vtg biosynthesis and its accumulation in the ovary is named “vitellogenesis”, and it is essential for ovarian maturation (Thongda et al, 2015)
Summary
Crustacea have different sexual systems, each aimed at achieving maximum reproduction efficiency in their specific situation. Ovary maturation comprises the synthesis of yolk protein denoted as Vitellin (Vn), which is the most important source of energy and nutrients for embryo development (Boulangé-Lecomte et al, 2017). The process of Vtg biosynthesis and its accumulation in the ovary is named “vitellogenesis”, and it is essential for ovarian maturation (Thongda et al, 2015). Vtg biosynthesis occurs in the ovary and/or hepatopancreas (Shen et al, 2014). There are differences between ovarian and extra-ovarian Vtg synthesis (Phiriyangkul et al, 2007). The Vtg gen, cDNA and protein sequence are fundamental for a molecular probe design to quantify the mRNA from the genes’ expression that participate in crustacean vitellogenesis
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