Crustacean female sex hormone: More than a female phenotypes-related hormone in a protandric simultaneous hermaphroditism shrimp

Abstract

Crustacean female sex hormone (CFSH) is believed to regulate the development of female-related phenotypes in crustaceans. However, its role in gonadal development has been understudied. This study identified a CFSH gene, Lvit-CFSH1b, in the peppermint shrimp Lysmata vittata, a protandric simultaneous hermaphroditism (PSH) species. Lvit-CFSH1b is only expressed in the eyestalk ganglion. qRT-PCR showed that the expression level of Lvit-CFSH1b significantly increased with the gonad development from stage I to III (male phase) and decreased at stage IV (euhermaphrodite phase). Gene knockdown of Lvit-CFSH1b resulted in retardation of female phenotypes and stimulated the development of male phenotypes. At the same time, ovarian development was inhibited, and spermatogenesis was promoted. In addition, injection of rCFSH1b increased ovarian expression of vitellogenin (Lvit-Vg) and hepatopancreas expression of vitellogenin receptor (Lvit-VgR), while suppressing the expressions of insulin-like androgenic gland hormones (Lvit-IAG1 and Lvit-IAG2) in androgenic glands. The addition of rCFSH1b induced the in vitro expression of Lvit-Vg in ovarian and Lvit-VgR in hepatopancreas explants. In conclusion, this study provides convincing evidence that CFSH expedites the feminization process and impedes masculinization by inhibiting IAG in hermaphroditic crustaceans.