Crude extract of the cyanobacterium, Hapalosiphon sp., causes a cessation of root elongation and cell division in several plant species

Abstract

The phytotoxic activities of the crude extract from the cyanobacterium, Hapalosiphon sp., on the initial growth and root cell division of several plant species were investigated. Although the germination percentages of the plants were not affected by the extract, their root and shoot growth were remarkably suppressed, depending on the extract concentrations. Roots were more sensitive to the extract than shoots and the sensitivity, determined by the I50 concentrations between the susceptible and tolerant species, differed by 5 and 11 times in dicotyledonous and monocotyledonous plants, respectively. The mitotic index for wheat (Triticum aestivum L. cv. Norin 61) root cells was not affected, but the mitotic index for onion (Allium cepa L. cv. Raputa II) was reduced with the increasing concentration of the extract. In the onion root meristem, the number of cells in prophase, metaphase, anaphase, and telophase were decreased significantly with 6 g DW L−1 (g dry weight of algae L−1). This suggests that the extract might inhibit the processes regulating the entry of cells into mitosis.