Abstract
Utilization of pineapple is always focused on consumption for fresh fruit and animal feed. many people do not know the other benefits of pineapple. The presence of protease enzymes in it, increasing its utilization. The enzyme is bromelin, which can be obtained by isolating it from pineapple. For this reason, the aim of this study is to isolate the crude bromelain enzyme based on the maturity level of pineapple and then test its proteolytic activity. Pineapple was blended, then the filtrate was deposited, then centrifuged to obtain crude bromelain which is dried by the drying method and tested for its activity using milk substrate. The results showed that old pineapple is the best level of maturity and the sun is the best drying method with proteolytic activity of 0.053 and 0.056 MCU/mg respectively. Its optimum activity is pH 5.8 and 55 oC.
Highlights
Utilization of pineapple is always focused on consumption for fresh fruit and animal feed. many people do not know the other benefits of pineapple
The results showed that old pineapple is the best level of maturity and the sun is the best drying method with proteolytic activity of 0.053 and 0.056 MCU/mg respectively
Journal of Food Science and Nutrition, 1(2), 40–46
Summary
Berdasarkan uraian di atas. enzim bromelin banyak dimanfaatkan dalam bidang pangan dan non-pangan. Kendala yang dihadapi untuk mengisolasi bromelin kasar dari buah nanas adalah penentuan teknik isolasi yang mudah untuk diterapkan. Berbagai teknik pemisahan dilakukan untuk memperoleh enzim bromelin dari buah nanas. Bromelin kasar dapat diekstrak dari limbah buah nanas Ekstrak nanas yang dimurnikan menunjukan aktivitas hemolitik yang dapat dikembangkan lebih lanjut dalam industri farmasi (Philip, dkk., 2016). Penelitian lainnya dilakukan dengan cara mengisolasi enzim bromelin kasar dari buah nanas. Hasil isolasi dan purifikasi secara parsial dari limbah batang buah nanas menunjukan aktivitas spesifik proteolitiknya tiga kali lebih tinggi (1.14 U/mg) dari pada sampel komersial bromelin (0.38 U/mg) yang memiliki jumlah protein total lebih tinggi (1.60 mg/mL) (Bresolin, dkk., 2013). Pada pH 9 masing-masing aktivitas proteolitik bromelin kasar dari batang dan kulit nanas adalah. Tulisan ini dimaksudkan untuk mengurai isolasi enzim bromelin kasar dengan tingkat kematangan yang berbeda pada berbagai metode pengeringan.
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