Abstract
Binding kinetics enhancement of a microfluidic biosensor into a micro-channel through the application of a supplementary mechanism has received tremendous attention because of the obtained significant enhancement factor. However, biosensor’s performance enhancement using only simple channel engineering is still rarely realized. Herein, we present a novel design of a complex reactive protein (CRP) biosensor into a U-shaped channel with a sensitive membrane located in the middle of the bent zone. Various critical factors affecting the equilibrium binding time are numerically investigated. The turn geometry is then optimized when the arc length along the inner and outer radii is almost the same, which leads to locally minimizing the channel height overhead the reaction surface and improves the analyte transport towards the sensing area. The numerical studies reveal that applying a local narrowing above the reaction surface can notably enhance the trapping and the surface formation of complex antibody-antigen, thus upgrading the biosensor performance. This work puts a significant advance towards microfluidic channel engineering and the exploration of micro-flow injection experimental studies.
Highlights
Over the past decades, the U-shape tube has been widely used in measurement [1] [2]
We present a novel design of a complex reactive protein (CRP) biosensor into a U-shaped channel with a sensitive membrane located in the middle of the bent zone
The numerical studies reveal that applying a local narrowing above the reaction surface can notably enhance the trapping and the surface formation of complex antibody-antigen, upgrading the biosensor performance
Summary
The U-shape tube has been widely used in measurement [1] [2]. One of the oldest measurements is pressure measurement, where the free end of the tube is reported with the atmosphere, and the pressure measurement is brought to another end [3]. A similar channel shape has been used for label-free detection of E. coli in which the designed biosensor exhibits a good sensitivity in solution measurements [14]. This shape showed enhanced outcomes with a 10-fold improvement in the absorbance sensitivity compared to the straight probes [15]. There is an ultimate need for the development of a rapid and highly sensitive biosensor for CRP detection, where the existence of an enhancement system can put a significant stepping stone for upgrading the biosensor performance. We find that upgrading the binding reaction without any supplementary enhancement mechanism via only simple channel engineering could have a significant role to fabricate microfluidic gadgets
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