Crown-of-thorns starfish predation and physical injuries promote brown band disease on corals

Abstract

Brown band (BrB) disease manifests on corals as a ciliate-dominated lesion that typically progresses rapidly causing extensive mortality, but it is unclear whe- ther the dominant ciliate Porpostoma guamense is a pri- mary or an opportunistic pathogen, the latter taking advantage of compromised coral tissue or depressed host resistance. In this study, manipulative aquarium-based experiments were used to investigate the role of P. gua- mense as a pathogen when inoculated onto fragments of the coral Acropora hyacinthus that were either healthy, preyed on by Acanthaster planci (crown-of-thorns starfish; COTS), or experimentally injured. Following ciliate inoc- ulation, BrB lesions developed on all of COTS-predated fragments (n = 9 fragments) and progressed up to 4.6 ± 0.3 cm d -1 , resulting in *70 % of coral tissue loss after 4 d. Similarly, BrB lesions developed rapidly on experimentally injured corals and *38 % of coral tissue area was lost 60 h after inoculation. In contrast, no BrB lesions were observed on healthy corals following experi- mental inoculations. A choice experiment demonstrated that ciliates are strongly attracted to physically injured corals, with over 55 % of inoculated ciliates migrating to injured corals and forming distinct lesions, whereas ciliates did not migrate to healthy corals. Our results indicate that ciliates characteristic of BrB disease are opportunistic pathogens that rapidly migrate to and colonise compro- mised coral tissue, leading to rapid coral mortality, par- ticularly following predation or injury. Predicted increases in tropical storms, cyclones, and COTS outbreaks are likely to increase the incidence of coral injury in the near future, promoting BrB disease and further contributing to declines in coral cover.