Abstract
Cellular crowding has wide-ranging significance on biomolecular processes inside the cell. In this study, the interaction of a charged polypeptide with a transmembrane protein pore was investigated using single-molecule electrophysiology under high concentrations of relatively large and neutral polyethylene glycols (PEG). Here, we show that the presence of high concentration PEGs at semi-dilute regime strengthens the affinity of polypeptide-pore interaction by increasing the association rate constant and decreasing the dissociation rate constant. this outcome is in agreement with the lower rate of diffusion in a crowded environment and with the higher depletion-attraction force between the polypeptide and PEG-free transmembrane pore.
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