Crosstalk in the magnocellular system during osmotic stimulation of one supraoptic nucleus

Abstract

Neural connections linking the four magnocellular nuclei i.e.,. the paired supraoptic (SON) and paraventricular (PVN) nuclei, may contribute to the simultaneous and parallel changes in firing patterns of oxytocinergic neurons during reflex milk ejection. To investigate these neural connections in the absence of suckling, intranuclear release of oxytocin (OT) was stimulated by microdialysis of hypertonic CSF containing 1 M NaCl (HS-CSF) into the right SON area and glucose metabolism of both SONs and PVNs and the neural lobe of virgin and lactating (10–12 day) rats was mapped by the autoradiographic [ 14C]deoxyglucose (DG) method. OT in the microdialysates and in plasma, obtained before and after 80–90 min of dialysis with CSF or HS-CSF, was quantified by RIA. In both virgin and lactating rats, microdialysis of HS-CSF unilaterally into the SON area significantly ( p < 0.05) increased release of OT in the nucleus and into plasma, which was associated with enhanced ( p < 0.05) metabolic activity in the ipsilateral and contralateral SON and the neural lobe but not in either PVN. Compared with virgins, lactating rats were less active, had lower ( p < 0.05) glucose utilization in the hypothalamo-neurohypophysial system, and less ( p < 0.05) OT in plasma during microdialysis of HS-CSF into the SON area. The osmotic stimulus did not activate neural structures (suprachiasmatic and medial amygdaloid nuclei) near the SON in either hemisphere. Thus, neural connections or, less likely, transport of OT via the subarachnoid space, may function to recruit activation of cells in the contralateral SON following hypertonic stimulation of cells in the other SON.