Abstract
Early sex determination of dioecious plants is essential for successful genetic improvement and commercial cultivation. Male and female seabuckthorn plants are morphologically similar and cannot be distinguished prior to 3–4 years of growth, i.e., at the time of flowering. India has three species of seabuckthorn namely Hippophae rhamnoides, Hippophae salicifolia and Hippophae tibetana. Applicability of sex linked SCAR markers HrX1 and HrX2 developed for H. rhamnoides were tested in H. salicifolia and H. tibetana using H. rhamnoides as control. HrX1 produced female specific amplification in both H. salicifolia and H. tibetana while HrX2 did not amplify except for H. rhamnoides. Sequence based analysis of amplified SCAR marker products HrX1, HsX1 and HtX1 has shown homology with known plant acyl CoA ligases.
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