Abstract
Enzymatic methods can be effective tools for removal of phenolic compounds from wastewater by forming reactive radicals with polymerization mechanism. Comparing with conventional chemical ways, enzymes have advantages such as low cost and high level of catalytic efficiency. At the same time, there exist some problems, e.g. the instability of enzyme structures, sensitivity to process conditions and their purification cost is high. Fine particles have been used for enzyme stabilization due to their beneficial high surface-to-volume ratio. Enzymes immobilized on fine particles may work at a broader pH, temperature and salinity range than the native enzymes. Ethyl cellulose and poly(lactic co-glycolic acid) carrier particles were prepared by nano spray drying and used for immobilizing horseradish peroxidase enzyme (HRP) by cross-linking. The optimal condition for immobilization was investigated. Fixed enzyme showed increased activity in TRIS buffer. The activity of the immobilized enzyme was enhanced at acidic and alkaline pH.
Highlights
Enzymes show a number of properties that make their use beneficial compared to conventional chemical ways, such as a high level of catalytic efficiency, high degree of substrate, regio- and stereospecificity
Ethyl cellulose and poly(lactic co-glycolic acid) carrier particles were prepared by nano spray drying and used for immobilizing horseradish peroxidase enzyme (HRP) by cross-linking
The aim of this work is the cross-linking of horseradish peroxidase enzyme to nano spray dried environmental friendly carrier particles such as ethyl cellulose (EC) and poly lactic coglycolic acid (PLGA), and preserving the enzyme activity at a broader pH and temperature range
Summary
Enzymes show a number of properties that make their use beneficial compared to conventional chemical ways, such as a high level of catalytic efficiency, high degree of substrate-, regio- and stereospecificity. This results in substantial process energy savings and decreased manufacturing costs [1]. There exist some problems in the use of enzymes such as isolation and purification costs are high, the instability of enzyme structures, sensitivity to process conditions and short lifetime often need to be solved. Immobilization can be a solution which can be achieved by fixing enzymes to or within solid supports stabilizing the structure of enzymes, their activities. Enzymes immobilized on fine particles may work at a broader pH, temperature and salinity range than the native enzymes [3]
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