Abstract
Cholesteryl group-modified tilapia gelatins (Chol-T-Gltns) with various Chol contents from 3 to 69 mol % per amino group of Gltn were prepared for the assembly of liposomes and cells. Liposomes were physically crosslinked by anchoring Chol groups of Chol-T-Gltns into lipid membranes. The resulting liposome gels were enzymatically degraded by addition of collagenase. Liposome gels prepared using Chol-T-Gltn with high Chol content (69Chol-T-Gltn) showed slower enzymatic degradation when compared with gels prepared using Chol-T-Gltn with low Chol content (3Chol-T-Gltn). The hepatocyte cell line HepG2 showed good assembly properties and no cytotoxic effects after addition of 69Chol-T-Gltns. In addition, the number of HepG2 cells increased with concentration of 69Chol-T-Gltns. Therefore, Chol-T-Gltn, particularly, 69Chol-T-Gltn, can be used as an assembling material for liposomes and various cell types. The resulting organization can be applied to various biomedical fields, such as drug delivery systems, tissue engineering and regenerative medicine.
Highlights
Architecture of functional nano/micro elements for three dimensional structures is a generation of nanotechnology
We employed tilapia-derived gelatin for the modification with Chol groups because of its low denaturation temperature compared with porcine [26]
From the 1H-NMR measurement of Chol-T-Gltn, the distinguishing signal of the Chol group was observed at 0.65 ppm (1H, 18C) after the reaction, indicating that the Chol group was introduced into the T-Gltn molecule
Summary
Architecture of functional nano/micro elements for three dimensional structures is a generation of nanotechnology. Liposomes have been used as a simple cell model and have been applied as drug carriers with broad clinical utility. There have been numerous approaches for assembling cells employing various techniques and substrates, such as bioreactors [17,18,19], culture-plates [20] and microfabricated surface-controlled cell adhesion [21]. These require special devices and techniques, and require long periods of time for assembly. We designed a novel biodegradable amphiphilic polymer that can assemble liposomes and cells. Assembly of hepatic cells using Chol-T-Gln was performed
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