Crossbridge viscosity in activated frog muscle fibres

Abstract

Force responses to fast ramp stretches at various velocities were recorded in single muscle fibres isolated from tibialis anterior muscle of the frog ( Rana esculenta) at a sarcomere length between 2.15 and 3.25 μm at 15°C. Stretches were applied at the tetanus plateau and during tetanus rise. Length changes were recorded at the sarcomere level using either a laser diffractometer or a striation follower apparatus. The immediate force response to the stretch is not simply elastic, as is usually assumed, but is composed of the sum of at least two components: (i) elastic (force proportional to the amount of stretch); and (ii) viscous (force proportional to the rate of stretch). The viscous response is associated with a short (about 10 μs) relaxation time. The amplitude of the viscous component increases progressively with tension during the tetanus rise and scales down with sarcomere length approximately in the same way as the tetanic tension. These results suggest that the viscosity of activated fibres may arise from crossbridge kinetics.